strong texthello everybody,
I'm doing for the first time an analysis of differential gene expression. already perform the alignment with tophat, then use samtools sort for the reads and now I want to count the readings with the HTSeq-count.
htseq-count -f bam -r name filesorted.bam hg19_genes.gtf > directoryoutput/file.HTSeq-count.txt
I get a message that says the following:
**0 GFF lines processed** **Warning: no features of type 'exon' found**
and the output file that generates have not name of the genes.
I would appreciate any help or guidance.