I see the following FastQC results in the "Per tile sequence quality" module:
Compared to other threads and articles I have seen, this does not look so bad in comparisson, but I am not sure if I should try to fix it anyway, files have been already run through Trimmomatic for quality trimming and adapter removal.
Samples come from a Hiseq4000 machine.
Libraries were prepared using TruSeq Nano chemestry.
Could you give me some advise on how to proceed with these samples?
Also, I cannot get the y-axis: according to Illumina, there are 896 tiles in a flow cell. Are tiles binned (or skipped) by FastQC?
I appreaciate your contributions