Question: How can we customize a SILVA reference database?
0
gravatar for vincent.carrier
8 days ago by
vincent.carrier0 wrote:

Hi,

the questions may sound obvious, but I am having difficulties to find a technique on how to customize the original fasta file of SILVA database that contains Archaea, Bacteria and Eukaryota to get three fasta file for each Domain (for instance here the newer release of SILVA 132). Thereafter, I would like to build files https://mothur.org/wiki/Silva_reference_files such as described here in the release 102 where for each domain I would obtain an aligned sequence file (e.g. silva.bacteria.fasta), an unaligned sequence file (e.g. nogap.bacteria.fasta), and taxonomy outlines (e.g. silva.bacteria.silva.tax).

I apologize it may sound simple, but any help would be highly appreciated!

Thanks!

amplicons silva • 62 views
ADD COMMENTlink modified 8 days ago by doctor.dee00570 • written 8 days ago by vincent.carrier0
0
gravatar for doctor.dee005
8 days ago by
Bioinformatics Center, Pune
doctor.dee00570 wrote:

If you have original SILVA db fasta file, you can simply extract sequences and their taxonomy(from taxonomy file) using grep(using Archaea, Bacteria and Eukaryota as keywords or expression) command and print them in separate files as you wish.

ADD COMMENTlink written 8 days ago by doctor.dee00570
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