Question: Pacbio-NGS, QIIME2, bbtools
gravatar for khulan1017
19 months ago by
khulan10170 wrote:

Hello, I am new to NGS, and I got the NGS data from Pacbio and I am trying to use QIIME2 but I have a problem with inputing data? My data is paired-end-sequence. In my understood, I have to create Metadata first, but how to create metadata with long -sequencing data? Also Pacbio fastq is different from Illumina and I tried to reformat fromASCII-33 to ASCII64 with using BBmaps, but still got the error. Is there have any tutorial for analysing Pacbio-NGS data? oh aslo, my data is bacterial amd fungal sequence data? Thanks!

sequencing next-gen • 766 views
ADD COMMENTlink modified 19 months ago by Carambakaracho2.0k • written 19 months ago by khulan10170

Hi khulan1017, your question is not as clear as you might think. Based on your goal to use qiime2, I guess you're trying to analyse amplicon sequencing data? Why would you have mixed bacterial and fungal sequences? What is the goal you're trying to achieve? What did you amplify?

These questions are important, because based on what you're goal is either I'm on the wrong track or there might be quite some limitations in data analysis due to the high error rate in PacBio data.

ADD REPLYlink written 19 months ago by Carambakaracho2.0k

Pacbio paired-end sequencing data? As far as I know that does not exist.

ADD REPLYlink written 19 months ago by WouterDeCoster43k
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