Off topic:How to extract reads belonging to sense intron/exon and antisense intron/exon while working with small RNA sequence
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2.7 years ago
toralmanvar ▴ 910

I have Illumina data of 1 x50bp from small RNA library. I am interested in identifying known and novel miRNAs in my plant, whose reference genome is not available. However, first I want to count the reads aligning Exon sense, Exon antisense, Intron sense, Intron antisense. Basically, I want to replicate the information shown in table below. I have gone through the complete publication and they have mentioned the use of Rfam database in "Experimental procedures" section for identifying mRNA. So my question is how can I classify reads as Exon sense, Exon antisense, Intron sense, Intron antisense using Rfam or any other databases? enter image description here

sRNA exon sense exon antisense intron sense • 844 views
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