Entering edit mode
5.8 years ago
aacharya
▴
10
Hi I am running script to read the MoGene-2_0-st.CEL file but unfortunately the error is coming
"Error in ReadAffy() : could not find function "ReadAffy".
Can any one please help how i can read :CEL file and perform rma.
#########CONSTRUCTING ESETs WITH .CEL FILES THROUGH AFFY###############
source("http://bioconductor.org/biocLite.R")
biocLite("affy")#Installs Affy i
library(affy)#Deploys Affy Package
setwd("C:/Users/akp0396//RTest cell file/Helene CEL file")#Define in folder
data<-ReadAffy()#Automatically reads the .CEL files present in the folder
data<-rma(data)
Error in ReadAffy() : could not find function "ReadAffy"
B-also I have tried by deploying "oligo" but then also i am getting error message which is as
oligo:::getCelChipType("C:/Users/akp0396//RTest cell file/Helene CEL file", TRUE)
Error in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]) :
namespace ‘BiocGenerics’ 0.26.0 is already loaded, but >= 0.27.1 is required
Hello and welcome aacharya,
Please use the formatting bar (especially the
code
option) to present your post better. I've done it for you this time.Thank you!
fin swimmer
Hello fin swimmer,
The tip is really helpful :)
Best Regards Avi
Which R version are you using?; What happened after you ran
library(affy)
? Also note the error on BiocGenericsI am using R version 3.5.1 (2018-07-02) -- "Feather Spray". BiocGenerics is loaded with affy:
Hello aacharya!
It appears that your post has been cross-posted to another site: Cross-posted at https://support.bioconductor.org/p/111334/
This is typically not recommended as it runs the risk of annoying people in both communities.
Hi Kevin, I am a new user and I am learning so expect experts like you may handle with experience :) while ran the
library(affy)
I am getting the ErrorReadAffy() : could not find function "ReadAffy"
I am still searching appropriate scripts to read and perform rma.Thanks for your support
Regards Avi
Did you try the solution by James on Bioconductor? - https://support.bioconductor.org/p/111334/#111377
Yes! but unfortunately after performing that, the problem didn't solve . so I removed
'oligo'
and I reinstalllibrary(oligo) with packages("Rcpp")
then after that I can able read and perform the RMA.Thanks for all the information and support :)
Best Regards Aviseka