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5.7 years ago
madhul27_sit
•
0
Hi, I have a data of silkworm (some rare species) whose genome is not reported yet. Thus I am doing De novo RNA seq analysis of it. I have followed Trinity protocol and when I perform RSEM for abundance estimation, it is giving me 50, 46, 51, 44 , 47 and 57 percent overall alignment rate, respectively, for each sample. Ideally, above 70% overall alignment rate is considered best. So, Does that mean there is a problem with data or should I try some other method for alignment and abundance estimation?
I have two samples with 3 biological replicates each.
By "followed the Trinity protocol", you mean you assembled the transcriptome with all the reads, then mapped each sample onto this reference transcriptome? What were the commands you used?