I am currently writing a project that would involve gene expression of different non-model animals. Until now I've been working with Illumina, generating RNA-Seq transcriptomic libraries and de novo assembling them with Trinity to have a reference transcriptome. This time I want to use Oxford Nanopore Technologies (ONT) MinION sequencer but I cannot find any assembler specific for de novo transcriptome assembly with just ONT long reads (I did find some genome assemblers). - Is there any? - I've been finding a lot of info about Hybrid-Seq, combining Illumina+ONT reads. Is this currently the only option to generate a de novo transcriptome using ONT?
Thanks in advance!