Excuse me, everybody. I am a new one here and also this field. I need help a lot .T-T
Parts of my story... I downloaded SRR__.sra file from ncbi using sra toolkit. i used fastq-dump to convert SRR__.sra to fastq file and splitted file into 3 files : 1.SRR____1.fastq 2.SRR____2.fastq 3.SRR___.fastq which is the combined version of 1+2
My question are.... 1) If i don't misunderstand, SRR___1.fastq and SRR___2.fastq are from the paired-end sequencing,right?
2)How can i merge the two file together? i want a single file to do the downstream process like mapping etc.
3)How can i get a file as a directly downloading file from ncbi website using sratoolkit? Was the directly downloading file already merged? (In the file, every sequence has optional description after seq name)
Please give me some suggestions. Thank you you guys in advance!