Question: (Closed) ChIA-PET with two antibodies
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gravatar for mkh
18 months ago by
mkh40
USA/CT
mkh40 wrote:

Hi All,

Is it possible to do ChIA-PET exp. with two different Antibodies at two steps? Imagine we have a complex with two proteins, proteinA and proteinB. Each of these proteins can interact with many different places in the genome but the complex of the proteinA and B will interact to fewer places relative to each of them separately. So I am interested to know , in ChIA-PET process, if we can use antibody of protein A to select the segment bound by protein A first, then use the second Antibody to select those DNA section that already interact with proteinA-Antobody complex? Thanks

ADD COMMENTlink written 18 months ago by mkh40

Hello morteza.khabiri!

We believe that this post does not fit the main topic of this site.

This is a wet-lab, rather than a bioinformatics question. Better post it on SeqAnswers or StackExchange.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink modified 18 months ago • written 18 months ago by ATpoint29k
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