Check with this fig, I'm so confused about these
I think signal generation step is not belong to macs2, since it is refer to mapping result, mapping let us know generated signal.
And peak calling is a function of macs2 but not mark here, actually, I can't tell the difference between peak calling and signal generation? For this fig, they need same input, bam or sam, right?
Which step's result can be as input to IGV? I used fold change over control bigwig files as input, worked, but some slices says after peak calling?
- I just have fold change over control and signal p-value result bigwig files now, so if I need to do peak calling, I must get mapping results bam or sam? Can't transfer bigwig files as input, right？ I mean information equivalence is not problem? Some tips, thanks！
I got figure from this websitehttps://www.encodeproject.org/chip-seq/transcription_factor/