Question: monocle clustering cells without marker genes error
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gravatar for hsu
7 days ago by
hsu0
hsu0 wrote:

I am using monocle to analyse my gene_counts matrix.

I am trying to use function :clustering cells without marker genes. And I am following the guideline on the website.

My code is :

HSMM <- reduceDimension(HSMM, max_components = 2, num_dim = 6,  reduction_method = 'tSNE', verbose = T)

The error is :

Remove noise by PCA ...
Reduce dimension by tSNE ...
Error in Rtsne.default(as.matrix(topDim_pca), dims = max_components, pca = F,  : 
  Remove duplicates before running TSNE.

what should I do about it? I appreciate your help.

rna-seq tool monocle R • 104 views
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