Question: Sequence specific bias for reverse transcriptase
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gravatar for rnaseqbias
7 days ago by
rnaseqbias0
rnaseqbias0 wrote:

Are there any known sequence-specific bias for reverse transcriptase? I am interested in the bias that may arise in the first strand cDNA synthesis step of RNA-Seq library preparation. Thank you.

sequencing rna-seq • 56 views
ADD COMMENTlink written 7 days ago by rnaseqbias0

Yes there are:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896536/

ADD REPLYlink written 7 days ago by h.mon18k
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