Closed:StringTie giving different results - same dataset

Question

[compuTE]

Hello,

I made two transcript assemblies with the same data set (same bam files) with StringTie, and I am getting somehow different results. Both times I got 223,641 transcripts in total, but the first time I ran it I got around ~40,000 de novo transcripts, and the second I got ~50,000.

I would like to know why. What is changing or is random in the algorithm?

Thank you,