Closed:StringTie giving different results - same dataset
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3.3 years ago
compuTE ▴ 90

Hello,

I made two transcript assemblies with the same data set (same bam files) with StringTie, and I am getting somehow different results. Both times I got 223,641 transcripts in total, but the first time I ran it I got around ~40,000 de novo transcripts, and the second I got ~50,000.

I would like to know why. What is changing or is random in the algorithm?

Thank you,

StringTie transcript assembly RNAseq denovo • 298 views
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