Difference between differential transcript expression and differential splicing
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3.8 years ago
sneha108ss ▴ 30

Hi, Is there a difference between differential transcript expression and differential splicing? To me both seem the same

transcript splicing expression • 2.4k views
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To me, in differential splicing analysis you are always interested in studying different isoforms, while in case of differential transcript expression you also can be interested in assessing expression levels of the same transcript across different conditions.

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3.7 years ago

I would say "differential splicing" is for a splicing event (for example, coverage for an exon and/or junction, or frequencies for specific parts of the isoforms). I think JunctionSeq is a nice option for splicing analysis (although, with that definition, DEXSeq, MATS, and MISO are also other popular options).

I would say "differential transcript" is for differences in full-length transcripts. For Illumina data, these would be estimates, where most programs that I can think of (Salmon, cufflinks, etc.) would have some strategy for assigning multi-mapped reads. If you have replicates, I think this can help in situations where there is greater variability in the estimates (for example, some papers show that estimates are less accurate for genes with more isoforms), but "differential gene" analysis using unique reads in unambiguous regions would be another option (using a program like htseq-count).

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3.8 years ago
h.mon 34k

Differential splicing, or more commonly alternative splicing, is the biological process in which a gene can code for more than one protein, by inclusion or exclusion of different exons on the final messenger RNA. These alternative mRNA are called transcript isoforms, each isoform will result in a different protein.

Differential transcript expression is the statistical analysis where one compares the expression of transcript isoforms between conditions.

The take away is: differential splicing is a biological process, differential transcript expression is an analytical method.

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