Hi There I am doing an edgeR analysis using RobiNA software. I have three types of samples (1,2 and 3) each type has multiple biological replicates. all the samples counts are included in one file. I am trying to find the differential expression genes between samples types 1 vs 3, and 2 vs 3. my question is when I change the number of the biological replicates of the sample type 1, the analysis results ( deferentially expressed genes ) of 2 vs 3 are different, even that nothing has been changed in the samples type 2 and 3. is this normal or I am doing something wrong. and it would be appreciated if someone can include an explanation for this.