Question: Merging WGS SNP array data
0
gravatar for sankar2004
4 months ago by
sankar200410
sankar200410 wrote:

Hi

I combined SNP array and WGS data and plotted a PCA. I found that the individuals from the same population did not cluster together because the SNVs were obtained from the two different (genotyping) methods mentioned above. How do you remove this bias/discrepancy from these datasets.

Thanks for your help

snp • 159 views
ADD COMMENTlink modified 4 months ago by RamRS20k • written 4 months ago by sankar200410
1

Thanks a lot Kevin. I will try that

ADD REPLYlink written 4 months ago by sankar200410
0
gravatar for Kevin Blighe
4 months ago by
Kevin Blighe35k
Republic of Ireland
Kevin Blighe35k wrote:

With your array data, you will have to filter out variants not called on the coding (+ / plus) strand, and then also filter these out of the NGS WGS data. Take a look at what I have written for Step 6, here: Produce PCA bi-plot for 1000 Genomes Phase III - Version 2

You can download the library files from the array manufacturer for the purposes of determining on which strand each probe genotypes.

Kevin

ADD COMMENTlink written 4 months ago by Kevin Blighe35k
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