Hi guys, I have a question about how to choose a reasonable threshold to define genes to be expressed (not differentially expressed but simply expressed) to compare gene expression measurements from different platforms. In other words I have to check if my list of genes is expressed using an RNA seq dataset, using a data set of one-color microarrays and another of two colours microarrays. If for RNA Seq is generally "accepted" (but I know that it depends on the experimental condition) an rpkm >1 I don't know how to set a threshold for microarrays. Any suggestion about this?
Thank you in advance