Hi everyone,
I conducted RT-qPCR to validate my transcriptome analysis. In my transcriptome data, the genes are expressed in Stage 1, but are not expressed in Stage 2. When I conducted qPCR, I was able to see the same pattern. However, I am facing problem when trying to analyze my data because my Cq value for Stage 2 is zero.How do I calculate my fold change for qPCR when my Cq values for Stage 2 is zero. I've used the readily available excel template and got huge positive number. Appreciate all the help I can get! Thank you!
Well, if it's zero, you can't show a fold change. Better off just showing the Cq values, gets the same idea cross. Just be sure to show them for your reference gene as well.
Edit: Devon's answer is an even better idea.
Thank you so much for the suggestion!