Question: Multi-part TCGA BAMs?
gravatar for lucas.lochovsky
7 months ago by
lucas.lochovsky0 wrote:

I'm working with TCGA WGS BAMs through the Google Cloud Platform, and I've seen that there are files where the barcode is identical, but there is an additional number appended to the filename before the .bam extension. For example,





Do these correspond to multiple parts of one BAM that should be merged before analysis? Or is this something else?

cancer bam data • 235 views
ADD COMMENTlink modified 6 months ago by Kevin Blighe41k • written 7 months ago by lucas.lochovsky0
gravatar for Kevin Blighe
6 months ago by
Kevin Blighe41k
Guy's Hospital, London
Kevin Blighe41k wrote:

These 2 are tumour, based on the part that I have put in bold:

  • G32450.TCGA-FD-A3N5- 01 A-11D-A21A-08.1.bam
  • G32450.TCGA-FD-A3N5- 01 A-11D-A21A-08.3.bam

These 2 are from normal tissue:

  • G32450.TCGA-FD-A3N5- 10 A-01D-A21A-08.2.bam
  • G32450.TCGA-FD-A3N5- 10 A-01D-A21A-08.4.bam

All of these, however, are from the Broad Institute, as judged by the final number 08,

Unless otherwise stated, the assumption, based on the 'rules' of naming samples, is that these are from the same aliquot (1 tumour aliquot; 1 normal aliquot).

Check the BAM [SAM] headers to see if they have also been processed in the same way. The commands used for alignment should be encoded in the header. In some situations, I've spotted TCGA BAMs from the same project aligned to different genomes.


ADD COMMENTlink written 6 months ago by Kevin Blighe41k
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