Sorry if this is a dumb question, but I am told by our sequencing core that you cannot detect methylation status of PCR amplicons with PacBio? Is that merely because the amplicons won't retain the methylated nucleotides? I was looking at the Nature Methods paper that is cited for this method of detection and I think they do use PCR amplification of custom fosmids prior to PacBio sequencing though.
Question: CpG Methylation data from PacBio sequencing
2.3 years ago by
nchuang • 250
nchuang • 250 wrote:
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