The following graph shows a fastq file (WGS) after trimming (sample comes from mouse). Adapters and low quality bases were removed with trimmomatic. The module "Overrepresented sequences" was flagged with a "PASS".
I have gone through other threads. This one in particular help me link1, but I am not sure what it means when a curve has "Small shoulders".
This other thread Link2 also gives useful hints, but again the meaning of "a broad secondary peak" can be ambiguous.
Based on the figure below, could you help me decide if the sample is acceptable for downstream analyses, and if I should apply some other tool to correct this issue?
EDIT: links fixed and sample nature added.