Question: Coverage Depth Transcriptome Assembly
gravatar for babji2318
18 months ago by
babji231810 wrote:

I am trying to estimate coverage depth for my de novo transcriptome assembly. I used Illumina Hiseq 3000/4000 PE with a PF yield of 140 million clusters. Since the organism hasn't been studied before I am having trouble finding the total number of transcripts. Can someone tell me of a way to calculate the coverage depth. Will I have to calculate the coverage depth on average for each transcript or can I get a coverage depth for the entire transcriptome? Thank you

rna-seq next-gen assembly • 461 views
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