Entering edit mode
5.5 years ago
babji2318
▴
10
I am trying to estimate coverage depth for my de novo transcriptome assembly. I used Illumina Hiseq 3000/4000 PE with a PF yield of 140 million clusters. Since the organism hasn't been studied before I am having trouble finding the total number of transcripts. Can someone tell me of a way to calculate the coverage depth. Will I have to calculate the coverage depth on average for each transcript or can I get a coverage depth for the entire transcriptome? Thank you
