I'm using the ubuntu app to install and launch ncbi-blast-2.7.1+ on Windows 10 and I am struggling as I've been following instructions from the following tutorials: https://www.blaststation.com/intl/members/en/howtoblastwin.html https://github.com/enormandeau/ncbi_blast_tutorial/blob/master/README.md and none of them seem to be helping me work.
I'm new to BLAST but I'm working on an assignment for which I need a BLAST database. I've installed blast-2.7.1 and it is located in C:/Program Files/ NCBI/blast-2.7.1 and I ran the program as instructed. I then created a directory (mkdir c/blast/db) where I downloaded ncbi-blast-2.7.1+-x64-win64.tar.gz and extracted the file. The FASTA file I'm using is called reference.fasta and I tried the following command:
makeblastdb -in reference.fasta -title reference -out reference -dbtype nucl
I got the following error
Command 'makeblastdb' not found, but can be installed with:
sudo apt install ncbi-blast+
I tried to use sudo apt instal ncbi-blast+ but it didn't work
Reading package lists... Done Building dependency tree Reading state information... Done E: Unable to locate package ncbi-blast
I am not sure what I am doing wrong.
I am also not sure what the following means in the tutorial:
Add the bin folder from the extracted archive to your path. For example, add the following line to your ~/.bashrc file: export PATH="/PATH/TO/ncbi-blast-2.3.0+/bin":$PATH And change the /PATH/TO part to the path where you have put the extracted archive.