We found a gene (TSC2) mutated with low VAF (around 7%) in 3/4 brain tumor patients. Although the sample size is limited, 3/4 patients is hard to be concidence. Besides, this gene is known to be a tumor suppressor preventing cells from growing too fast.
However, the low VAF makes it less unlikely to be a tumor suppressor (not a truncal mutation).
We found such case a bit contradictory. Can anyone share some comments on this and what can we do to make furthur investigation? Thanks in advance.