Question: primers for whole metagenome
0
gravatar for M.Nadeem Khan
4 months ago by
Pakistan/Islamabad/Quaid-I-Azam University
M.Nadeem Khan0 wrote:

Hello All I want to do amplify all the bacterail genomic contents present in environmental samples. What kind of primers I have to use? Primers used for 16s are enough for this procedure? or I have to desgin special type of primers?

sequencing genome • 228 views
ADD COMMENTlink modified 10 weeks ago by Fabio Marroni2.0k • written 4 months ago by M.Nadeem Khan0
1

Primers used for 16s are enough for this procedure?

No

ADD REPLYlink written 4 months ago by biokoala3.6k
1

This is not a bioinformatics question. Anyway, buy some multiple displacement amplification kit (just be aware of its shortcomings)..

ADD REPLYlink modified 4 months ago • written 4 months ago by 5heikki8.1k
1

This is just shotgun sequencing right? So no use of PCR

ADD REPLYlink written 4 months ago by gb590
1

It's a step you perform prior to any sequencing

ADD REPLYlink written 4 months ago by 5heikki8.1k
1
gravatar for Fabio Marroni
10 weeks ago by
Fabio Marroni2.0k
Italy
Fabio Marroni2.0k wrote:

I am sorry for this shameful self-promotion, but you can give a look at the methods section of our recent paper (actually still waiting for reviewers!) in which the library preparation for WGS is explained.

However, I agree with @gb that being shotgun sequencing by definition you must avoid using primers for amplifying any given region.

ADD COMMENTlink written 10 weeks ago by Fabio Marroni2.0k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 817 users visited in the last hour