Which normalization is good for gene expression classification and clustering
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2.5 years ago
Sara ▴ 60

Hi All, I have gene expression (raw count and FPKM) from healthy and disease states. I'm going to do supervised and unsupervised classification on gene expression. As dimension reduction I'm going to get differential expression genes from DESeq2 and use these genes as features in my classification.
The problem is that I don't know which normalization is good in the classification study? Is it good to use FPKM? What about using DESeq2 normalization?

I would appreciate if someone could advise me, Many thanks

classification FPKM Deseq2 Normalization • 846 views
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2.5 years ago
ATpoint 48k

rlog and vst are recommended options. Check the DESeq2 manual for details.

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so many thanks for your reply

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