Question: Differential expression analysis using GFOLD and DESeq2
gravatar for newbio17
23 months ago by
newbio17240 wrote:

Details about the experiment can be found on bioconductor page below:

Bioconductor post

The author of DESeq2 mentioned that I cannot get analysis results for interaction between type and time due to the number of samples.

Since GFOLD is specifically designed for doing analysis without replicates, could I present results from GFOLD for differential expression between type and time interaction alongside with DESeq2 results?

Thank you.

gfold rna-seq deseq2 • 557 views
ADD COMMENTlink modified 23 months ago by Kevin Blighe65k • written 23 months ago by newbio17240
gravatar for Kevin Blighe
23 months ago by
Kevin Blighe65k
Kevin Blighe65k wrote:

Sure you can, but just be very clear about how you derived the different statistical values. Be as 'brutally honest' about your analysis as you possibly can, and be prepared for criticism. For one, I would never give much credibility to comparisons containing just a single sample. There may be logical reasons why you have not been able to increase your sample n, though.


ADD COMMENTlink written 23 months ago by Kevin Blighe65k
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