Hello guys, new to the club.
I'm interested in intersecting my AcH3k27 peaks with some enhancers signature (or known enhancers) in mouse fibroblasts.
I have a couple of questions in this regard:
1) How much are enhancers signatures "Stable" across cell lines? I can understand AcH3k27 changing (Because is relative to enhancer activation) but I cannot see why Enhancers signatures should actually change between different cell lines. I am not talking about signal magnitude but the mere presence of the signature.
2) Other than H3K4me1, how can one identify and annotate enhancers? Have they been annotated? I do have a good ChIP-seq for AcH3k27 and a NA-seq for this primary mouse cells and I'm very curious to have a look at Enhancers role in my experimental settings. If you would have only this 2 dataset at hand, how would you take enhancers into consideration?
Thanks a lot;