Question: How accurate is UniProt?
6 months ago by
alexille5640 • 10
alexille5640 • 10 wrote:
I am curious about the reliability of Uniprot amino acid sequences?
Say I want to do the following:
- Reverse translate a reviewed Uniprot amino acid sequence into a nucleotide sequence (using Sequence Manipulation Suite Reverse Translate)
- Synthesize that nucleotide sequence in vitro into an expression vector plasmid
- Transfect cells with that plasmid for expression
Is it expected that a protein with the exact same amino acid sequence will be expressed by the cells?
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