Different questions about non-coding RNA, assemble genes and gene amplification

Question

[jesus-bn]

with what technique could I know if an RNA is non-coding?

How could you know the size of a genome before amplifying it?

We all know that there are many programs to study the assembly of genes, but which are really good criteria to check it?

Thanks!

[h.mon]

You want to know which genes are coding and non-coding in an annotated or unannotated genome?

You can estimate genome size with kmer profiles (see a tutorial here and here, or search the web for more details), or using flow cytometry. Of course, genome size estimation with kmer profiles depends on generating sequencing data.