Question: (Closed) reason of synthesizing the second strand of cDNA
gravatar for dude74775
5 months ago by
dude747750 wrote:

Hello everyone. I have been studying about RNA-Seq and I have been wondering why do we synthesize the second strand of cDNA just to degrade it with dUTP method and why can't we just use the first strand right away if we are sequencing only the first strand anyways? now I know it's a pretty basic question but I wasn't able to find any explanations to it online. thanks in advance

ADD COMMENTlink written 5 months ago by dude747750

Hello dude74775!

We believe that this post does not fit the main topic of this site.

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ADD REPLYlink written 5 months ago by WouterDeCoster38k
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