Question: Error correction of long reads
2
gravatar for IP
13 days ago by
IP500
Denmark/University of Copenagen
IP500 wrote:

Hi,

I am going to get some data from plasmid sequencing to identify SNPs on the plasmids. What it is done on the lab is the following:

  • The plasmids are purified by size
  • We amplify the plasmids using the phi29 polimerase. The polimerase will go trough the plasmid multiple times. Hence we get the same sequence concatenated multiple times. My question is related to this step
  • We sequence it using Oxford Nanopore

My question is:

On step two, I wrote that "we get the same sequence concatenated multiple times". For me, this a potential source of information to correct the base calls prior to aligning them to the reference. Since you have the same sequence multiple times ( concatemers). What I would like to know is if there is a tool that uses the information of the concatemers to improve the base calls. I have try to find some methods but found none. I know that PacBio has a similar flavour using the "circular consensus calling", But I have not found any methodological explanation.

thanks!

sequencing pacbio nanopore • 81 views
ADD COMMENTlink modified 10 days ago • written 13 days ago by IP500

I am posting it here since this is not answered

ADD REPLYlink modified 11 days ago • written 11 days ago by IP500
2
gravatar for IP
10 days ago by
IP500
Denmark/University of Copenagen
IP500 wrote:

Thanks to this answer in bioinformatics stackexchange, there is a tool doing exactly what you want. The tool is called C3P0a

ADD COMMENTlink modified 10 days ago • written 10 days ago by IP500
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