Question: What's the meaning of the strand+ or strand-?
0
gravatar for fffuuue
2.2 years ago by
fffuuue0
fffuuue0 wrote:

I'm a new explorer in circRNA. I want to get the sequence of a circRNA. But I find my circRNA is different from the other circRNA! Just like the picture, the most circRNA is strand+,but my circRNA is strand- . I'm wondering the meaning of strand + or strand -. I hope someone can solve my question. Thanks

strand

rna-seq circrna • 828 views
ADD COMMENTlink modified 2.2 years ago by Erin_Ensembl410 • written 2.2 years ago by fffuuue0

See How to add images to a Biostars post to add your images properly. You need the direct link to the image, not the link to the webpage that has the image embedded (which is what you have used here)

ADD REPLYlink written 2.2 years ago by _r_am32k
3
gravatar for Erin_Ensembl
2.2 years ago by
Erin_Ensembl410
EMBL-EBI
Erin_Ensembl410 wrote:

Yes this is where the gene is located on the DNA strand. DNA is made of two separate 'strands' that are complementary to each other and make up the DNA double helix. The strands are referred to as positive/forward strand, or negative/reverse strand. This other Biostars post answers it in good detail: Forward And Reverse Strand Conventions

ADD COMMENTlink written 2.2 years ago by Erin_Ensembl410
2
gravatar for ATpoint
2.2 years ago by
ATpoint44k
ATpoint44k wrote:

The strand information specifies if an RNA has been transcribed from either the top (+) or bottom (-) strand of the template DNA. This is a very basic biology question, and I strongly recommend to get some background in it before continuing your analysis.

ADD COMMENTlink written 2.2 years ago by ATpoint44k
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