Question: Paired-End Data Only Has One Read Per Spot
0
gravatar for lemmonquiche
7 months ago by
lemmonquiche0 wrote:

I am exploring some data generated using drop-seq (SRR6261587). The data should contain paired-end reads and the layout agrees. Yet there is only one read per spot as indicated by both the metadata and reads. How would I generate the paired-end format from this or am I missing something?

Thanks in advance.

drop-seq • 199 views
ADD COMMENTlink modified 7 months ago by jayeshkumarsundaram0 • written 7 months ago by lemmonquiche0

Problem: "Pair-end" reads scRNA seq data (Drop-seq)

Did you find out why?

ADD REPLYlink written 7 months ago by jayeshkumarsundaram0
0
gravatar for swbarnes2
7 months ago by
swbarnes25.8k
United States
swbarnes25.8k wrote:

The entry for this experiment in ENA agrees; paired data, just one read. Looking at the file, they didn't smuggle the data from read 2 in there anywhere, or interleave the reads together.

ADD COMMENTlink written 7 months ago by swbarnes25.8k
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