Primer Design for amplifying complete viral genome

Question

[babasaraki]

Hi All,

I am just wondering if there is a way we can design a set of say four sets of primers pairs or five sets that will amplify a 15kb genome size of a virus.

Any idea or recommendations/guide will gladly be appreciated.

Thank you

[Michael]

Comment from the wet lab: Make sure you use a suitable polymerase for the resulting fragment size, normal Taq polymerase can possibly not amplify this fragment size.

Answer 1

[WouterDeCoster]

That looks like a job for Primal Scheme.