I did an RNA seq and then I did the normalization of TMM. Afterwards I chose:
- 1 contig which was over-expressed
- my 4 plants populations
- the TMM values regarding this specific contig and the 4 populations.
So I thought to do an ANOVA test to check for any st.sign. diff. among the populations for the specific contig. In the end I did a t test with Bonferroni adjustment.
Do you think that this whole thing I did make sense? If not what you could recommend to me?
Btw I tried the heatmap but I think my excel set up is somehow wrong...
Thank you very much in advance!