I've gone over a couple of previous questions discussing a similar subject and they seem to all imply that it simply is not feasible to do with unstranded RNA-Seq data.
We know that for RNA-Seq, RNA is reverse transcribed into cDNA and that antisense RNA is complementary to its mRNA on the sense strand. If we were interested in detecting antisense RNA for a given gene, can't we just look for a complementary sequence of the gene (e.g. first 20 sequences)?
I understand this could be a problem since we don't know whether the matching sequence from the RNA-Seq reads is actually coming from the antisense strand, but how likely is it to detect, for example, a sequence of length 20, on the sense strand by chance?
Are there any additional things that should be taken into consideration?