Question: Primer-blast impossible with multiple sequences ?
1
gravatar for Picasa
23 months ago by
Picasa560
Picasa560 wrote:

Hi Biostars,

I am trying to design primers. For this I have multiple sequences in a fasta file and I have found this program that give kind of nice visualisation:

https://www.ncbi.nlm.nih.gov/tools/primer-blast/

1) However I cannot paste multiples sequences .. Is it normal or I am doing something wrong ?? I got this error:

Exception error: Multiple templates are currently not supported! .

2) A command line version does'nt exist ?

3) If no, do you have any recommendation for other programs that take multiple input and/or can be run by command line ?

Thanks a lot.

primer-blast sequences • 1.4k views
ADD COMMENTlink modified 23 months ago by Biostar ♦♦ 20 • written 23 months ago by Picasa560

Past thread that may be of interest: Automated Primer-Blast like funcionnality

ADD REPLYlink written 23 months ago by genomax91k

Thanks but it is not what I am looking for. This thread talks about finding DNA sequences from a primer pair, while I want the reverse (finding primer pair from dna sequence)

ADD REPLYlink modified 23 months ago • written 23 months ago by Picasa560

You can use Primer3 in the commad-line.

https://sourceforge.net/projects/primer3/

ADD REPLYlink written 23 months ago by h.mon31k
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