how to find the UMi position?
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5.4 years ago
DNAseq • 0

Hi, In my library, the insert is ligated to an adaptor sequence that contains the first index and the UMI. My question is: the UMI will be in read 1, read 2 or both? in 5' or 3' end? I did a paired end sequencing 150pb. Thank you,

alignment UMI • 1.4k views
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