I'm an undergrad taking a genomics course where I'm doing a mini research project on a gene (APOE). For one of my hypothesis, I would like to compare the expression of APOE between different organisms and see what the differences are and if it is significant. Eventually I want to also compare the folding as well and see what the differences in the transport functions are. I'm lacking in the genetics and genomics subject (and really regretting taking this course) so I'm a bit unsure of how to execute this and obtain this data?
My thoughts were to possibly obtain the cDNA (humans) and run a BLAST (blastx?) against the other organisms and compare the values from there? I'm not really sure though if this is correct and what values would be significant for what I'm looking for (query?).
If anyone knows anything about this, please give some guidance! Anything at all will help, even literature recommendations on something similar to what I am doing would be tremendous help!