Question: Align two single cell experiments
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elb • 160 wrote:
Hi guys, I'm trying to align two single cell experiments using the Seurat package. According to the tutorial here: https://satijalab.org/seurat/Seurat_AlignmentTutorial.html the standard pipeline first performs the FindVariableGenes step and then creates an object from the union of the two datasets. I would like to perform the regression on mt-genes and on cell cycle genes. My question is: when to perform the regression? On the single objects independently before the union or on the object from the union?
Thank you in advance!
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modified 10 months ago
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Michael Dondrup ♦ 46k
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10 months ago by
elb • 160