Question: Align two single cell experiments
1
gravatar for elb
4 months ago by
elb160
Torino
elb160 wrote:

Hi guys, I'm trying to align two single cell experiments using the Seurat package. According to the tutorial here: https://satijalab.org/seurat/Seurat_AlignmentTutorial.html the standard pipeline first performs the FindVariableGenes step and then creates an object from the union of the two datasets. I would like to perform the regression on mt-genes and on cell cycle genes. My question is: when to perform the regression? On the single objects independently before the union or on the object from the union?

Thank you in advance!

single cell job R • 249 views
ADD COMMENTlink modified 4 months ago by Michael Dondrup46k • written 4 months ago by elb160
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