Align two single cell experiments
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5.6 years ago
elb ▴ 250

Hi guys,

I'm trying to align two single cell experiments using the Seurat package. According to the tutorial here: https://satijalab.org/seurat/Seurat_AlignmentTutorial.html the standard pipeline first performs the FindVariableGenes step and then creates an object from the union of the two datasets. I would like to perform the regression on mt-genes and on cell cycle genes. My question is: when to perform the regression? On the single objects independently before the union or on the object from the union?

Thank you in advance!

R single-cell • 1.4k views
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