Entering edit mode
5.4 years ago
Dr.Animo
▴
130
Hi, I want to assemble the illumina reads by using unicycler. So, what parameters should I set for better assembly. I ran a example reads, which are alredy assemble. I want to compare the results. I set the default parameters, but the difference between result is very large. So, can you help me? 1. Exclude contigs shorter than (100) 2. The expected number of linear (0) 3. SPAdes options? a. Lowest k-mer size? b. Highest k-mer? c. Exact k-mers ? d. Number of k-mer steps ? e. Filter out contigs lower than this fraction of chr depth ?
