Berlin, 11-15 February 2019
Application deadline is: January 10th, 2019.
Assistant instructor: Mr. Maximilian Driller (Begendiv, Germany; http://bit.ly/2zcwmQT)
This course will introduce biologists and bioinformaticians to the concepts of de novo assembly and annotation. Different technologies, from Illumina, PacBio, Oxford Nanopoor and maybe 10X will be used mixed with different approaches like correction, HiC scaffolding to generate good draft assemblies. Particular attention will be given to the quality control of the assemblies and to the understanding how errors occur. Further, annotation tools using RNA-Seq data will be introduced. An outlook of potential analysis is given. In the end of the course the students should be able to understand what is needed to generate a good annotated genome.
Target Audience & Assumed Background
- Understand the concepts and quality of de novo assembly and annotation for genomes of all sizes, virus to mammals
- Learn the advantages of the different sequencing technologies e.g. Illumina, Pacific Bioscience and Oxford Nanopore for de novo assembly and how to access the quality of genomes sequences
- Hands on experience of common tools for de novo sequence assembly, including visualization, contig ordering, scaffolding and error correction
- Hands on experience of gene finding, including the use of RNA-Seq data
- Being comfortable to assemble and annotate genomes
For more information about the course, please visit our website: https://www.physalia-courses.org/courses-workshops/course20/
Here is the full list of our courses and Workshops: https://www.physalia-courses.org/courses-workshops/