Question: STAR alignment error: quantmod not found
0
gravatar for prabin.dm
8 days ago by
prabin.dm30
prabin.dm30 wrote:

Hi all,

I am using STAR for aligment and gettting an error. I can't figure out why?

star 2.5.3a is located under /share/pkg/star/2.5.3a
/home/.lsbatch/1544131842.9569914.shell: line 22: --quantMode: command not found

Here is my code

module load star/2.5.3a

STAR -- genomeDir star_genome_mm10 \
        --readFilesCommand zcat \
        --readFilesIn L001_R1_001.fastq.gz \
        --outSAMtype BAM SortedByCoordinate \
        --outSAMunmapped Within \
        --twopassMode Basic \
        --outFilterMultimapNmax 1 \ 
        --quantMode TranscriptomeSAM GeneCounts \
        --runThreadN 6 \
        --outFileNamePrefix /STAR_output/test

Another issue I am having is, the output files are not stored in the STAR_ouput directory. It is stored in the directory where the code is executed ?

star alignment rnaseq • 110 views
ADD COMMENTlink modified 8 days ago by Devon Ryan86k • written 8 days ago by prabin.dm30

Maybe change this

--outFileNamePrefix /STAR_output/test

to

--outFileNamePrefix $PWD/STAR_output/test

unless there's actually a dir named STAR_output with proper permissions in the root directory?

ADD REPLYlink written 8 days ago by RamRS19k
2
gravatar for Devon Ryan
8 days ago by
Devon Ryan86k
Freiburg, Germany
Devon Ryan86k wrote:

-- genomeDir has a space in it.

ADD COMMENTlink written 8 days ago by Devon Ryan86k

correcting that resulted in the same output. Should I have the rsem-prepare-reference run before I use --quantMode TranscriptomeSAM ?

ADD REPLYlink modified 8 days ago • written 8 days ago by prabin.dm30

Your command has a random space or a missing \ somewhere that you're not showing us. Write it all on one line without any \.

ADD REPLYlink written 8 days ago by Devon Ryan86k
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