Question: How to proceed after Trinotate?
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gravatar for Ismaelrymy
6 months ago by
Ismaelrymy0
Ismaelrymy0 wrote:

Hi there.

I'm new to the field of transcriptomics, and, as such, finding information sometimes can be quite difficult. I have to assemble a reference transcriptome de novo, and i have made it through trimming, filtering, assembly, and almost annotation. I used Trinotate to annotate my first draft of the transcriptome, but now, I don't know how to proceed.

I have a .csv file, with all the blast results (and other annotations), but I don't know how to filter this information (one blast result has several transcripts, some of them complete, some fragmentary) and I would like to know how would you proceed in this step of the analysis.

If you have some kind of input or resources to check online I would be very grateful.

ADD COMMENTlink modified 6 months ago by Giovanni.madrigal1280 • written 6 months ago by Ismaelrymy0
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gravatar for Giovanni.madrigal12
6 months ago by
Giovanni.madrigal1280 wrote:

You may consider predicting GO terms for your transcripts or evaluating the completeness of your transcriptome using BUSCO (https://busco.ezlab.org/). Additional details of your experiment (e.g. species of interest) may aid in getting better advice on this subject.

ADD COMMENTlink written 6 months ago by Giovanni.madrigal1280
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