Question: How to rectify mirdeep.pl error?
0
gravatar for nivya.james2016
22 months ago by
nivya.james20160 wrote:

Hi all,

Could you help me rectify this error?

mkdir mirdeep_runs/run_13_12_2018_t_16_20_32
#testing input files
#testing input files
started: 16:20:33
sanity_check_mature_ref.pl /home/shanthi/Desktop/Nivya/miRBase/mature_human_miRNA1.fa
Error: problem with /home/shanthi/Desktop/Nivya/miRBase/mature_human_miRNA1.fa
Error in line 5.309: The sequence
contains characters others than [acgtunACGTUN]
Please check your file for the following issues:
I.  Sequences are allowed only to comprise characters [ACGTNacgtn].
II. Identifiers are not allowed to have withespaces.

Thanking You in Advance

mirdeep2.pl • 334 views
ADD COMMENTlink modified 22 months ago by finswimmer13k • written 22 months ago by nivya.james20160
1

Hello,

how does /home/shanthi/Desktop/Nivya/miRBase/mature_human_miRNA1.fa look at line 5309?

You see this easily with sed -n '5309p' /home/shanthi/Desktop/Nivya/miRBase/mature_human_miRNA1.fa

fin swimmer

ADD REPLYlink written 22 months ago by finswimmer13k
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