I tried to use the deeptool ComputeGCBias with a sorted BAM file as Input. I used a BAM file directly from BWA mapping, a filtered BAM file (SAM tools, Filter SAM or BAM), and an additionally sorted BAM file. I always get the same error code that I cannot explain.
Fatal error: Exit code 1 () Traceback (most recent call last): File "/usr/local/tools/_conda/envs/mulled-v1-4b8f57f006cc92d851cc49218dae7aad45ffa09ba66fc274bc342ee27143a74c/bin/computeGCBias", line 11, in <module> main(args) File "/usr/local/tools/_conda/envs/mulled-v1-4b8f57f006cc92d851cc49218dae7aad45ffa09ba66fc274bc342ee27143a74c/lib/python3.6/site-packages/deeptools/computeGCBias.py", line 657, in main tbit = py2bit.open(global_vars['2bit']) RuntimeError: Received an error during file opening!
I appreciate your help so much, thank you!