Entering edit mode
5.3 years ago
gingerplum
•
0
I have a question regarding --ss and --exon parameters of HISAT2. Memory of my server is very limited, thus I have to use pre-built index. It seems that I can supply the lists of splice sites and exons of up-to-date gene annotation to --ss and --exon parameters. What is the difference between rebuilding index and playing around with the above two parameters? Any ideas? Thanks very much!