a question regarding --ss and --exon parameters of HISAT2
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5.3 years ago
gingerplum • 0

I have a question regarding --ss and --exon parameters of HISAT2. Memory of my server is very limited, thus I have to use pre-built index. It seems that I can supply the lists of splice sites and exons of up-to-date gene annotation to --ss and --exon parameters. What is the difference between rebuilding index and playing around with the above two parameters? Any ideas? Thanks very much!

RNA-Seq alignment • 978 views
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