Hi, I want to use "primer3_core" to design PCR primers from a multi-fasta file.
The --help says : $ primer3_core < my_input_file
But output said :
PRIMER_ERROR=thermodynamic approach chosen, but path to thermodynamic parameters not specified
Maybe it has something to do with the "PRIMER_THERMODYNAMIC_PARAMETERS_PATH", but the doc is not clear enough for me. There is also no clear example of a command line.